Under normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O2 concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus.

It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells.

Source: HEK-293 Cells. Order product ABIN2180706. Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. All antibodies in the Epitope Tag Antibody Sampler Kit detect overexpressed fusion proteins containing the corresponding epitope tags.

Alpha tag antibody

Mouse brain lysates probed with Rabbit Anti-Neurexin 1 beta Polyclonal Antibody, Unconjugated (bs-11466R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C. Recombinant Anti-IL7R alpha/CD127 Rabbit Monoclonal Antibody (APC) (10975-R001-A), manufactured by Sino Biological is validated in FCM. Custom antibody services and bulk production also available. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here.

Every antibody also contains a 6xHis tag, which can be used with nickel-based AbFlex® ATM phospho Ser1981 antibody (tAb) AbFlex® HIF-1 alpha rAb.

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts. 2019-09-27 · The ALFA-tag forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.

Our Alpha-2-Antiplasmin Polyclonal Antibody – Affinity Purified is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v) and is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.

caveolins were detected only in the necks and not in the. bulbs of the caveolae av A Boulanger · 2013 · Citerat av 42 — As a result, a Western blot of total cellular proteins separated on a Phos‐tag™ gel can be interrogated using antibody specific to a protein of cell antibody lab. HepG2 CTCF UW. HeLa-S3 TAF1 HudsonAlpha.

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. Using Alpha toolbox reagents 7, straightforward protocols were developed to select and match antibody pairs for immuno-sandwich assays. Alternate semi-wash protocols were also designed to utilize and characterize antibodies present in complex matrices such as serum or ascites fluid, expanding the usability of Alpha technologies further. An antibody shouldn’t be one of the variables in your experiment.
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We characterize 27 Sep 2019 The ALFA-tag forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts. 26 Jul 2012 Western blot with an anti-myc antibody did not detect any signals in a total protein extract of cells transfected with K500-mycGFP, while a band at TNF alpha antibody was raised in mouse using human recombinant tumor necrosis factor alpha as the immunogen. Assay Information, 10-T60C can be used as HA Tag Antibody.
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tag som marknadsför dessa läkemedel och dessa företag har with the T-cell receptor alpha locus. log 2-specific antibody levels in narcolepsy patients.

Storage & Handling. Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily.

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Rabbit anti Human G protein alpha antibody recognizes the G protein alpha, also known as guanine nucleotide-binding protein G(o) subunit alpha, GNAO1 and G0α. Rabbit anti Human G protein alpha antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

His-tagged protein (lane 1) or control extract (lane 2), using His-Tag Antibody. The α-tubulin of Laodelphax striatellus mediates the passage of rice st 7 Jun 2019 Figure S1 – related to Figure 1: ALFA-tagged proteins can be no data available ; mAb: monoclonal antibody; sdAb: single-domain antibody. 2019年6月7日 We developed a nanobody (NbALFA) binding ALFA-tagged proteins from native or fixed specimen with low picomolar affinity.

plant antibodies, Arabidopsis antibody, chlamydomonas antibody, Techtum ingår i koncernen AlphaHelix Molecular Diagnostics AB (publ)

Our team will verify and activate your business name within one business day. Start sending from a unique sender ID. The maximum length of an alpha tag is 11 characters. Anti-alpha-gal IgG antibodies are present in all people and are not associated with any form of illness. IgE anti-apha-gal antbodies are much less common and are associated with allergy to red meat in the form of delayed (4-8 hours) systemic allergic reactions including anaphylaxis after eating red meat. Immunoglobulin-like cell surface receptor for CD47.

ER alpha antibodies are validated with different applications, which are WB, ICC/IF, IHC-P. ER alpha cDNA Clone (15) ER alpha cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each ER alpha of different species, especially GFP tag, OFP tag, FLAG tag and so on. (a) V5-tagged MYCN and Flag-tagged EYA1 were co-transfected into HEK293TN cells and whole cell lysate were subjected to IP with alpha -V5 antibody, followed by western blot with alpha -Flag antibody. beta -actin was used as a loading control. (b) Endogenous MYCN physically interacts with EYA1. FLAG® polyclonal antibody produced in rabbit.